PrimePCR™ Probe Assay: Sult2a2, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0043490
Assay Design:   Intron-spanning
Chromosome Location:   7:13733507-13734784question
Amplicon Length:   196
Splice Variants Targeted:   ENSMUST00000086148 ENSMUST00000108522 ENSMUST00000098809 ENSMUST00000076576

Gene Information

This is one of seven sulfotransferase family 2A genes in a chromosome 7 A1 cluster. [provided by RefSeq May 2010]

Gene Symbol:   Sult2a2
Gene Name:   Sulfotransferase family 2A, dehydroepiandrosterone (DHEA)-preferring, member 2
Aliases:   C730007P19Rik, ST, ST2A2, Sth2, mSTa2
RefSeq:   NC_000073.6 NT_187034.1
Ensembl:   ENSMUSG00000070811
Entrez:   100043194
UniGene:   Mm.457986
Chromosome Mapping:   7 A1|7

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 41.240000
Efficiency 81

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download