PrimePCR™ Probe Assay: Olfr682-ps1, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Olfr682-ps1, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Olfr682-ps1, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0043293
Assay Design:   Intron-spanning
Chromosome Location:   7:105126891-105128237question
Amplicon Length:   181
Splice Variants Targeted:   ENSMUST00000106080 ENSMUST00000106079 ENSMUST00000030588 ENSMUST00000057254 ENSMUST00000071242 ENSMUST00000061284 ENSMUST00000005600 ENSMUST00000114731 ENSMUST00000082290 ENSMUST00000178164 ENSMUST00000025109 ENSMUST00000107938 ENSMUST00000086693 ENSMUST00000097291 ENSMUST00000145347 ENSMUST00000006629 ENSMUST00000102838 ENSMUST00000124231 ENSMUST00000011877 ENSMUST00000036748 ENSMUST00000087897 ENSMUST00000119705 ENSMUST00000049780 ENSMUST00000120902 ENSMUST00000044011 ENSMUST00000142271 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Olfr682-ps1
Gene Name:   Olfactory receptor 682, pseudogene 1
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSMUSG00000059768
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 36.050000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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