PrimePCR™ Probe Assay: Rpl38, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0043248
Assay Design:   Intron-spanning
Chromosome Location:   11:114668950-114671845question
Amplicon Length:   99
Splice Variants Targeted:   ENSMUST00000088904 ENSMUST00000047510 ENSMUST00000122110 ENSMUST00000106602 ENSMUST00000077915 ENSMUST00000106599 ENSMUST00000082092

Gene Information

Description Not Available

Gene Symbol:   Rpl38
Gene Name:   Ribosomal protein L38
Aliases:   0610025G13Rik, Rbt, Ts, Tss
RefSeq:   NC_000077.6 NT_096135.6
Ensembl:   ENSMUSG00000057322
Entrez:   67671
UniGene:   Mm.238817
Chromosome Mapping:   11 79.82 cM|11 G1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 37.010000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download