PrimePCR™ Probe Assay: Xlr, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0043221
Assay Design:   Intron-spanning
Chromosome Location:   X:53787911-53796153question
Amplicon Length:   169
Splice Variants Targeted:   ENSMUST00000067782 ENSMUST00000067763 ENSMUST00000114810 ENSMUST00000033458

Gene Information

Description Not Available

Gene Symbol:   Xlr
Gene Name:   X-linked lymphocyte-regulated complex
Aliases:   Not Available
RefSeq:   NC_000086.7 NT_111910.3
Ensembl:   ENSMUSG00000054626
Entrez:   22441
UniGene:   Mm.379041
Chromosome Mapping:   X A5|X

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 37.610000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download