PrimePCR™ Probe Assay: Fbxw14, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0043216
Assay Design:   Intron-spanning
Chromosome Location:   9:109285297-109286533question
Amplicon Length:   167
Splice Variants Targeted:   ENSMUST00000054925 ENSMUST00000120329 ENSMUST00000066901 ENSMUST00000112041 ENSMUST00000119470 ENSMUST00000112040 ENSMUST00000112039 ENSMUST00000080626 ENSMUST00000084984 ENSMUST00000076617 ENSMUST00000056745 ENSMUST00000163839 ENSMUST00000071917

Gene Information

Description Not Available

Gene Symbol:   Fbxw14
Gene Name:   F-box and WD-40 domain protein 14
Aliases:   AU045528, E330009N23Rik, Fbx12, Fbxo12
RefSeq:   NC_000075.6 NT_039482.8
Ensembl:   ENSMUSG00000054087
Entrez:   50757
UniGene:   Mm.296059
Chromosome Mapping:   9 F2|9

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 37.660000
Efficiency 91

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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