PrimePCR™ Probe Assay: Znrf1, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0043107
Assay Design:   Intron-spanning
Chromosome Location:   8:111619215-111621648question
Amplicon Length:   120
Splice Variants Targeted:   ENSMUST00000172856 ENSMUST00000095176 ENSMUST00000173506 ENSMUST00000173726 ENSMUST00000174454 ENSMUST00000174333 ENSMUST00000173781 ENSMUST00000171182 ENSMUST00000168428 ENSMUST00000166859 ENSMUST00000123128 ENSMUST00000029069 ENSMUST00000165922

Gene Information

Description Not Available

Gene Symbol:   Znrf1
Gene Name:   Zinc and ring finger 1
Aliases:   B830022L21Rik, Rnf42, Zrfp1, nin283
RefSeq:   NC_000074.6 NT_078575.7
Ensembl:   ENSMUSG00000033545
Entrez:   170737
UniGene:   Mm.271578
Chromosome Mapping:   8 E1|8

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 37.340000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download