PrimePCR™ Probe Assay: Gprin2, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Gprin2, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Gprin2, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0034499
Assay Design:   Intron-spanning
Chromosome Location:   14:34195709-34201511question
Amplicon Length:   181
Splice Variants Targeted:   ENSMUST00000096019 ENSMUST00000025243 ENSMUST00000049430 ENSMUST00000106663 ENSMUST00000025786 ENSMUST00000160424 ENSMUST00000160175 ENSMUST00000047923 ENSMUST00000056288 ENSMUST00000077472 ENSMUST00000080215 ENSMUST00000081857 ENSMUST00000021851 ENSMUST00000102803 ENSMUST00000168485 ENSMUST00000001672 ENSMUST00000174259 ENSMUST00000095806 ENSMUST00000120259 ENSMUST00000021467 ENSMUST00000059849 ENSMUST00000064447

Gene Information

Description Not Available

Gene Symbol:   Gprin2
Gene Name:   G protein regulated inducer of neurite outgrowth 2
Aliases:   C130040D06Rik, C230073P13, Gm286, mKIAA0514
RefSeq:   NC_000080.6 NT_039606.8
Ensembl:   ENSMUSG00000071531
Entrez:   432839
Chromosome Mapping:   14 B|14

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.100000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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