PrimePCR™ Probe Assay: Hsd11b1, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Hsd11b1, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Hsd11b1, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0033030
Assay Design:   Intron-spanning
Chromosome Location:   1:193240361-193241393question
Amplicon Length:   200
Splice Variants Targeted:   ENSMUST00000161737 ENSMUST00000016338 ENSMUST00000160929 ENSMUST00000159644 ENSMUST00000162842

Gene Information

Description Not Available

Gene Symbol:   Hsd11b1
Gene Name:   Hydroxysteroid 11-beta dehydrogenase 1
Aliases:   Not Available
RefSeq:   NC_000067.6 NT_039190.8
Ensembl:   ENSMUSG00000016194
Entrez:   15483
Chromosome Mapping:   1 H6|1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 34.850000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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