PrimePCR™ Probe Assay: St6gal1, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: St6gal1, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: St6gal1, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0032174
Assay Design:   Intron-spanning
Chromosome Location:   16:23328414-23356231question
Amplicon Length:   117
Splice Variants Targeted:   ENSMUST00000023601 ENSMUST00000115335 ENSMUST00000178797

Gene Information

This gene encodes a member of glycosyltransferase family 29. The encoded protein is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The protein which is normally found in the Golgi but can be proteolytically processed to a soluble form is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6 CD75 and CD76. This gene has been incorrectly referred to as CD75. Alternatively spliced transcript variants have been observed for this gene and a pseudogene of this gene is located on chromosome 15. [provided by RefSeq Nov 2011]

Gene Symbol:   St6gal1
Gene Name:   Beta galactoside alpha 2,6 sialyltransferase 1
Aliases:   AW742324, Siat1, St6Gal-I, St6gal, St6galI
RefSeq:   NC_000082.6 NT_039624.8
Ensembl:   ENSMUSG00000022885
Entrez:   20440
Chromosome Mapping:   16 B1|16 15.5 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 34.590000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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