PrimePCR™ Probe Assay: Pou2f1, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0029974
Assay Design:   Intron-spanning
Chromosome Location:   1:165902246-165906751question
Amplicon Length:   105
Splice Variants Targeted:   ENSMUST00000159212 ENSMUST00000160260 ENSMUST00000111429 ENSMUST00000160908 ENSMUST00000027850 ENSMUST00000069609 ENSMUST00000176942 ENSMUST00000111426 ENSMUST00000111427

Gene Information

Description Not Available

Gene Symbol:   Pou2f1
Gene Name:   POU domain, class 2, transcription factor 1
Aliases:   2810482H01Rik, NF-A1, Oct-1, Oct1, Otf-1, Otf1
RefSeq:   NC_000067.6 NT_039185.8
Ensembl:   ENSMUSG00000026565
Entrez:   18986
Chromosome Mapping:   1 H2.3|1 87.2 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.930000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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