PrimePCR™ SYBR® Green Assay: Lamtor3, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCIP0043736

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Lamtor3, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Lamtor3, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0041520
Assay Design:   Intron-spanning
Chromosome Location:   3:137918623-137926854question
Amplicon Length:   197
Splice Variants Targeted:   ENSMUST00000105393 ENSMUST00000118539 ENSMUST00000022099 ENSMUST00000179862 ENSMUST00000033575 ENSMUST00000025046 ENSMUST00000066149 ENSMUST00000050958 ENSMUST00000180037 ENSMUST00000177957 ENSMUST00000024879 ENSMUST00000002445 ENSMUST00000106770 ENSMUST00000045644 ENSMUST00000123325 ENSMUST00000110410 ENSMUST00000145798 ENSMUST00000179393 ENSMUST00000023510 ENSMUST00000038389 ENSMUST00000168345 ENSMUST00000112793 ENSMUST00000075591 ENSMUST00000078320 ENSMUST00000112790 ENSMUST00000112825 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Lamtor3
Gene Name:   Late endosomal/lysosomal adaptor, MAPK and MTOR activator 3
Aliases:   AW556229, Map2k1ip1, Mapbp, Mapksp1, Mp1
RefSeq:   NC_000069.6 NT_039240.8
Ensembl:   ENSMUSG00000091512
Entrez:   56692
Chromosome Mapping:   3 G3|3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.997500
y-intercept 39.990000
Efficiency 87

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download