PrimePCR™ SYBR® Green Assay: Gm12776, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCIP0043641

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Gm12776, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Gm12776, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0041425
Assay Design:   Intron-spanning
Chromosome Location:   7:34095431-34099788question
Amplicon Length:   197
Splice Variants Targeted:   ENSMUST00000159109 ENSMUST00000143238 ENSMUST00000085618 ENSMUST00000057910 ENSMUST00000108083 ENSMUST00000025003 ENSMUST00000056781 ENSMUST00000089049 ENSMUST00000079491 ENSMUST00000075387 ENSMUST00000169037 ENSMUST00000168545 ENSMUST00000171456 ENSMUST00000098521 ENSMUST00000058716 ENSMUST00000110884 ENSMUST00000078690 ENSMUST00000128402 ENSMUST00000050776 ENSMUST00000124059 ENSMUST00000113849 ENSMUST00000113847 ENSMUST00000169906 ENSMUST00000151986 ENSMUST00000107396

Gene Information

Description Not Available

Gene Symbol:   Gm12776
Gene Name:   Predicted gene 12776
Aliases:   Gm12776, OTTMUSG00000008394
RefSeq:   NC_000073.6 NT_187034.1
Ensembl:   ENSMUSG00000078752
Entrez:   100043868
Chromosome Mapping:   7 B1|7

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.710000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download