PrimePCR™ SYBR® Green Assay: Atp5h, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCIP0043111

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Atp5h, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Atp5h, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0040894
Assay Design:   Intron-spanning
Chromosome Location:   11:115416038-115417875question
Amplicon Length:   119
Splice Variants Targeted:   ENSMUST00000027512 ENSMUST00000113186 ENSMUST00000113190 ENSMUST00000090488 ENSMUST00000031732 ENSMUST00000111002 ENSMUST00000077309 ENSMUST00000043931 ENSMUST00000106537 ENSMUST00000073791 ENSMUST00000137754 ENSMUST00000180072 ENSMUST00000025718 ENSMUST00000030427

Gene Information

Description Not Available

Gene Symbol:   Atp5h
Gene Name:   ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d
Aliases:   0610009D10Rik
RefSeq:   NC_000077.6 NT_096135.6
Ensembl:   ENSMUSG00000034566
Entrez:   71679
Chromosome Mapping:   11 E2|11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.290000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download