PrimePCR™ SYBR® Green Assay: Dbndd2, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best specificity; Same primer pair as used in probe assay qMmuCIP0043020

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Dbndd2, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Dbndd2, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0040803
Assay Design:   Intron-spanning
Chromosome Location:   2:164488630-164490210question
Amplicon Length:   121
Splice Variants Targeted:   ENSMUST00000033289 ENSMUST00000047621 ENSMUST00000154873 ENSMUST00000114799 ENSMUST00000156862 ENSMUST00000080572 ENSMUST00000019363 ENSMUST00000025224 ENSMUST00000082254 ENSMUST00000115181 ENSMUST00000024987 ENSMUST00000159610 ENSMUST00000159048 ENSMUST00000161035 ENSMUST00000078496 ENSMUST00000107747 ENSMUST00000084616 ENSMUST00000107907 ENSMUST00000107906 ENSMUST00000035208 ENSMUST00000178687 ENSMUST00000084535 ENSMUST00000130411 ENSMUST00000035540 ENSMUST00000055506 ENSMUST00000015605 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Dbndd2
Gene Name:   Dysbindin (dystrobrevin binding protein 1) domain containing 2
Aliases:   1110017A21Rik, 2900022J10Rik, AU041050, AW048677, D2Bwg0891e, Nkip, R74724
RefSeq:   NC_000068.7 NT_039207.8
Ensembl:   ENSMUSG00000017734
Entrez:   52840
Chromosome Mapping:   2 H3|2 93.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 42.360000
Efficiency 80

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download