PrimePCR™ SYBR® Green Assay: Med16, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCIP0043013

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Med16, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Med16, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0040796
Assay Design:   Intron-spanning
Chromosome Location:   10:79903340-79906792question
Amplicon Length:   87
Splice Variants Targeted:   ENSMUST00000105378 ENSMUST00000165684 ENSMUST00000164705 ENSMUST00000166964 ENSMUST00000005749 ENSMUST00000087395 ENSMUST00000175660 ENSMUST00000176191 ENSMUST00000176978 ENSMUST00000045011 ENSMUST00000161521 ENSMUST00000053273 ENSMUST00000079573 ENSMUST00000131890 ENSMUST00000139732 ENSMUST00000169350 ENSMUST00000043676 ENSMUST00000098457 ENSMUST00000150503 ENSMUST00000105659 ENSMUST00000105658 ENSMUST00000135185 ENSMUST00000043464 ENSMUST00000068813 ENSMUST00000102521 ENSMUST00000047488 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Med16
Gene Name:   Mediator complex subunit 16
Aliases:   95kDa, A630083L04, Thrap5, Trap95
RefSeq:   NC_000076.6 NT_039500.8
Ensembl:   ENSMUSG00000013833
Entrez:   216154
Chromosome Mapping:   10 C1|10

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.700000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download