PrimePCR™ SYBR® Green Assay: Tmem247, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: Tmem247, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Tmem247, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0024363
Assay Design:   Intron-spanning
Chromosome Location:   17:86918427-86922253question
Amplicon Length:   198
Splice Variants Targeted:   ENSMUST00000092415 ENSMUST00000108709 ENSMUST00000023846 ENSMUST00000023818 ENSMUST00000156348 ENSMUST00000168381 ENSMUST00000086444 ENSMUST00000113371 ENSMUST00000040504 ENSMUST00000133447 ENSMUST00000113370 ENSMUST00000086617 ENSMUST00000073156 ENSMUST00000103109 ENSMUST00000039390 ENSMUST00000034862 ENSMUST00000024854 ENSMUST00000136390 ENSMUST00000002603 ENSMUST00000063747 ENSMUST00000109946 ENSMUST00000166086 ENSMUST00000028155 ENSMUST00000113869 ENSMUST00000113867 ENSMUST00000113870 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Tmem247
Gene Name:   Transmembrane protein 247
Aliases:   1700090G07Rik
RefSeq:   NC_000083.6 NT_039649.8
Ensembl:   ENSMUSG00000037689
Entrez:   78469
Chromosome Mapping:   17 E4|17

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998400
y-intercept 36.300000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download