PrimePCR™ SYBR® Green Assay: Alg2, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Alg2, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Alg2, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0020910
Assay Design:   Intron-spanning
Chromosome Location:   4:47472426-47474135question
Amplicon Length:   200
Splice Variants Targeted:   ENSMUST00000019268 ENSMUST00000039608 ENSMUST00000036003 ENSMUST00000051358 ENSMUST00000107284 ENSMUST00000107285 ENSMUST00000026537 ENSMUST00000179347 ENSMUST00000044148 ENSMUST00000041106 ENSMUST00000167588 ENSMUST00000107498 ENSMUST00000090942 ENSMUST00000081695 ENSMUST00000028665 ENSMUST00000059401 ENSMUST00000040162 ENSMUST00000108339

Gene Information

Description Not Available

Gene Symbol:   Alg2
Gene Name:   Asparagine-linked glycosylation 2 homolog (yeast, alpha-1,3-mannosyltransferase)
Aliases:   1110018A23Rik, 1300013N08Rik, ALPG2, CDGIi, MNCb-5081
RefSeq:   NC_000070.6 NT_187032.1
Ensembl:   ENSMUSG00000039740
Entrez:   56737
Chromosome Mapping:   4|4 B2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.530000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download