PrimePCR™ SYBR® Green Assay: Neurod2, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Neurod2, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Neurod2, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0020906
Assay Design:   Intron-spanning
Chromosome Location:   11:98328246-98329453question
Amplicon Length:   86
Splice Variants Targeted:   ENSMUST00000114621 ENSMUST00000033700 ENSMUST00000114617 ENSMUST00000171933 ENSMUST00000061970 ENSMUST00000025391 ENSMUST00000093138 ENSMUST00000101270 ENSMUST00000134021 ENSMUST00000043464 ENSMUST00000067268 ENSMUST00000086523 ENSMUST00000159236 ENSMUST00000159680 ENSMUST00000162777 ENSMUST00000161964 ENSMUST00000103201 ENSMUST00000020843 ENSMUST00000040695 ENSMUST00000123952 ENSMUST00000017851 ENSMUST00000049040 ENSMUST00000041685 ENSMUST00000110677 ENSMUST00000085684

Gene Information

Description Not Available

Gene Symbol:   Neurod2
Gene Name:   Neurogenic differentiation 2
Aliases:   Ndrf, bHLHa1
RefSeq:   NC_000077.6 NT_096135.6
Ensembl:   ENSMUSG00000038255
Entrez:   18013
Chromosome Mapping:   11 D|11 57.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.310000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download