PrimePCR™ SYBR® Green Assay: Tshz3, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Tshz3, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Tshz3, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0020851
Assay Design:   Intron-spanning
Chromosome Location:   7:36698219-36768716question
Amplicon Length:   130
Splice Variants Targeted:   ENSMUST00000095580 ENSMUST00000070139 ENSMUST00000137551 ENSMUST00000069817 ENSMUST00000021641 ENSMUST00000108506 ENSMUST00000138587 ENSMUST00000047441 ENSMUST00000161263 ENSMUST00000102919 ENSMUST00000066379 ENSMUST00000020490 ENSMUST00000090558 ENSMUST00000147251 ENSMUST00000045229 ENSMUST00000060835 ENSMUST00000075249 ENSMUST00000095487 ENSMUST00000178486 ENSMUST00000023741

Gene Information

Description Not Available

Gene Symbol:   Tshz3
Gene Name:   Teashirt zinc finger family member 3
Aliases:   A630038G13Rik, Tsh3, Zfp537, mKIAA1474, teashirt3
RefSeq:   NC_000073.6 NT_187034.1
Ensembl:   ENSMUSG00000021217
Entrez:   243931
Chromosome Mapping:   7 B2|7

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 36.300000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download