PrimePCR™ SYBR® Green Assay: Smad2, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0017003
Assay Design:   Intron-spanning
Chromosome Location:   18:76298448-76299832question
Amplicon Length:   74
Splice Variants Targeted:   ENSMUST00000168423 ENSMUST00000091831 ENSMUST00000172198 ENSMUST00000025453 ENSMUST00000165084 ENSMUST00000113930

Gene Information

Description Not Available

Gene Symbol:   Smad2
Gene Name:   MAD homolog 2 (Drosophila)
Aliases:   7120426M23Rik, Madh2, Madr2, Smad-2, mMad2
RefSeq:   NC_000084.6 NT_039674.8
Ensembl:   ENSMUSG00000024563
Entrez:   17126
UniGene:   Mm.152699
Chromosome Mapping:   18 E3|18 48.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.350000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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