PrimePCR™ SYBR® Green Assay: Itgb4, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCIP0033533

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Itgb4, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Itgb4, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0016597
Assay Design:   Intron-spanning
Chromosome Location:   11:115993407-115994337question
Amplicon Length:   96
Splice Variants Targeted:   ENSMUST00000021107 ENSMUST00000106460 ENSMUST00000068981 ENSMUST00000106458 ENSMUST00000106461 ENSMUST00000169928

Gene Information

Integrins are heterodimers comprised of alpha and beta subunits that are noncovalently associated transmembrane glycoprotein receptors. Different combinations of alpha and beta polypeptides form complexes that vary in their ligand-binding specificities. Integrins mediate cell-matrix or cell-cell adhesion and transduced signals that regulate gene expression and cell growth. This gene encodes the integrin beta 4 subunit a receptor for the laminins. This subunit tends to associate with alpha 6 subunit and is likely to play a pivotal role in the biology of invasive carcinoma. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   Itgb4
Gene Name:   Integrin beta 4
Aliases:   AA407042, C230078O20, CD104
RefSeq:   NC_000077.6 NT_096135.6
Ensembl:   ENSMUSG00000020758
Entrez:   192897
Chromosome Mapping:   11 E2|11 76.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.000000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download