PrimePCR™ SYBR® Green Assay: Sh3bp2, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $142.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0015959
Assay Design:   Intron-spanning
Chromosome Location:   5:34555955-34556929question
Amplicon Length:   67
Splice Variants Targeted:   ENSMUST00000067638 ENSMUST00000101316 ENSMUST00000118545 ENSMUST00000179943

Gene Information

Description Not Available

Gene Symbol:   Sh3bp2
Gene Name:   SH3-domain binding protein 2
Aliases:   3BP2
RefSeq:   NC_000071.6 NT_039305.8
Ensembl:   ENSMUSG00000054520
Entrez:   24055
UniGene:   Mm.5012
Chromosome Mapping:   5 B2|5

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 34.940000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download