PrimePCR™ SYBR® Green Assay: Nek8, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Nek8, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Nek8, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0007125
Assay Design:   Intron-spanning
Chromosome Location:   11:78173044-78176631question
Amplicon Length:   129
Splice Variants Targeted:   ENSMUST00000017549 ENSMUST00000148154

Gene Information

This gene encodes a NIMA-related kinase. Members of this serine/threonine protein kinase family are structurally-related to NIMA (never in mitosis gene A) which controls mitotic signaling in Aspergillus nidulans. [provided by RefSeq Jul 2008]

Gene Symbol:   Nek8
Gene Name:   NIMA (never in mitosis gene a)-related expressed kinase 8
Aliases:   4632401F23Rik, jck
RefSeq:   NC_000077.6 NT_096135.6
Ensembl:   ENSMUSG00000017405
Entrez:   140859
Chromosome Mapping:   11 B5|11 44.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 34.420000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download