This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qMmuCIP0028153
PrimePCR™ PreAmp for SYBR® Green Assay: Myc, Mouse
PrimePCR™ Template for SYBR® Green Assay: Myc, Mouse
The protein encoded by this gene is a multifunctional nuclear phosphoprotein that plays a role in cell cycle progression apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations overexpression rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors leukemias and lymphomas including Burkitt lymphoma in human. There is evidence to show that alternative translation initiations from an upstream in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini in human and mouse. Under conditions of stress such as high cell densities and methionine deprivation there is a specific and dramatic increase in the synthesis of the non-AUG initiated protein suggesting its importance in times of adversity. Alternative splicing results in multiple transcript variants. [provided by RefSeq Apr 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.