PrimePCR™ SYBR® Green Assay: Gsk3a, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $146.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0006179
Assay Design:   Intron-spanning
Chromosome Location:   7:25230844-25231839question
Amplicon Length:   121
Splice Variants Targeted:   ENSMUST00000071739 ENSMUST00000108411

Gene Information

Description Not Available

Gene Symbol:   Gsk3a
Gene Name:   Glycogen synthase kinase 3 alpha
Aliases:   2700086H06Rik
RefSeq:   NC_000073.6 NT_187034.1
Ensembl:   ENSMUSG00000057177
Entrez:   606496
UniGene:   Mm.294664
Chromosome Mapping:   7 A3|7

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.994100
y-intercept 34.090000
Efficiency 108

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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