PrimePCR™ SYBR® Green Assay: Trp73, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: Trp73, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Trp73, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0006017
Assay Design:   Intron-spanning
Chromosome Location:   4:154064673-154067041question
Amplicon Length:   77
Splice Variants Targeted:   ENSMUST00000133533 ENSMUST00000105644 ENSMUST00000097762 ENSMUST00000105643 ENSMUST00000155642

Gene Information

This gene encodes tumor protein p73 which is a member of the p53 family of transcription factors involved in cellular responses to stress and development. The family members include p53 p63 and p73 and have high sequence similarity to one another which allows p63 and p73 to transactivate p53-responsive genes causing cell cycle arrest and apoptosis. The family members can interact with each other in many ways involving direct or indirect protein interactions resulting in regulation of the same target gene promoters or regulation of each other's promoters. The p73 protein is expressed at very low levels in normal tissues and is differentially expressed in a number of tumors. The p73 gene expresses at least 35 mRNA variants due to the use of alternate promoters alternate translation initiation sites and multiple splice variations. Theoretically this can account for 29 different p73 isoforms; however the biological validity and the full-length nature of most variants have not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   Trp73
Gene Name:   Transformation related protein 73
Aliases:   Tp73, p73
RefSeq:   NC_000070.6 NT_187033.1
Ensembl:   ENSMUSG00000029026
Entrez:   22062
Chromosome Mapping:   4 E2|4 82.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 37.750000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download