PrimePCR™ Probe Assay: Zfp59, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   Recommended - best efficiency

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Zfp59, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Zfp59, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0062926
Assay Design:   exonic
Chromosome Location:   7:27853487-27853715question
Amplicon Length:   199
Splice Variants Targeted:   ENSMUST00000080175 ENSMUST00000108331 ENSMUST00000180024

Gene Information

The Krueppel-associated box (KRAB) is a domain of around 75 amino acids found in the N-terminal portion of about one third of eukaryotic Krueppel-type C2H2 zinc finger proteins (ZFPs). The KRAB domain functions as a transcriptional repressor when tethered to the template DNA by a DNA-binding domain. Although the function of KRAB-ZFPs is largely unknown they appear to play important roles during cell differentiation and development. This gene encodes a protein belonging to the Krueppel family of C2H2-type zinc finger proteins and has been shown to accumulate in the nuclei of mature sperm in association with the nuclear matrix. [provided by RefSeq Jul 2008]

Gene Symbol:   Zfp59
Gene Name:   Zinc finger protein 59
Aliases:   Mfg-2, Mfg2
RefSeq:   NC_000073.6 NT_187034.1
Ensembl:   ENSMUSG00000078779
Entrez:   22717
Chromosome Mapping:   7 A3|7

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.260000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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