PrimePCR™ Probe Assay: Golga2, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   Recommended - best coverage

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Golga2, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Golga2, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0062806
Assay Design:   exonic
Chromosome Location:   2:32288392-32292208question
Amplicon Length:   91
Splice Variants Targeted:   ENSMUST00000114017 ENSMUST00000114015 ENSMUST00000077355 ENSMUST00000114012 ENSMUST00000114018 ENSMUST00000024639 ENSMUST00000025951 ENSMUST00000112335 ENSMUST00000139494 ENSMUST00000113377 ENSMUST00000131712 ENSMUST00000133113 ENSMUST00000081670 ENSMUST00000147707 ENSMUST00000129193 ENSMUST00000100194

Gene Information

Description Not Available

Gene Symbol:   Golga2
Gene Name:   Golgi autoantigen, golgin subfamily a, 2
Aliases:   AW555139, GM130, mKIAA4150
RefSeq:   NC_000068.7 NT_039206.8
Ensembl:   ENSMUSG00000002546
Entrez:   99412
Chromosome Mapping:   2 B|2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.330000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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