PrimePCR™ Probe Assay: Vmn1r219, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   Recommended - best efficiency

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Vmn1r219, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Vmn1r219, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0062612
Assay Design:   exonic
Chromosome Location:   13:23162862-23162973question
Amplicon Length:   82
Splice Variants Targeted:   ENSMUST00000091739 ENSMUST00000075055 ENSMUST00000072369 ENSMUST00000091732 ENSMUST00000091731 ENSMUST00000091729 ENSMUST00000074324 ENSMUST00000110445 ENSMUST00000124841 ENSMUST00000095961 ENSMUST00000072044 ENSMUST00000095960 ENSMUST00000076897 ENSMUST00000074252 ENSMUST00000091721 ENSMUST00000076180 ENSMUST00000072385 ENSMUST00000077116 ENSMUST00000041105 ENSMUST00000116594 ENSMUST00000167713 ENSMUST00000175805 ENSMUST00000070143 ENSMUST00000177276 ENSMUST00000110168

Gene Information

Description Not Available

Gene Symbol:   Vmn1r219
Gene Name:   Vomeronasal 1 receptor 219
Aliases:   V1rh13
RefSeq:   NC_000079.6 NT_039578.8
Ensembl:   ENSMUSG00000061376
Entrez:   171272
Chromosome Mapping:   13 A3.1|13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 35.430000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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