PrimePCR™ Probe Assay: Cpne1, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Cpne1, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Cpne1, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0060630
Assay Design:   exonic
Chromosome Location:   2:156078408-156078649question
Amplicon Length:   60
Splice Variants Targeted:   ENSMUST00000096069 ENSMUST00000111062 ENSMUST00000022660 ENSMUST00000159840 ENSMUST00000160884 ENSMUST00000030768 ENSMUST00000071093 ENSMUST00000106283 ENSMUST00000057167 ENSMUST00000109608 ENSMUST00000109607 ENSMUST00000079312 ENSMUST00000136296 ENSMUST00000153634 ENSMUST00000142960 ENSMUST00000133921

Gene Information

This gene encodes a protein that contains two N-terminal type II C2 domains and an integrin A domain-like sequence in the C-terminus. Its activity is also upregulated in mouse embryos. This gene and the gene for RNA binding motif protein 12 overlap at map location 2 H2. Two alternatively spliced variants that encode the same isoform have been identified for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   Cpne1
Gene Name:   Copine I
Aliases:   1810028N16Rik, mKIAA4108
RefSeq:   NC_000068.7 NT_039207.8
Ensembl:   ENSMUSG00000074643
Entrez:   266692
Chromosome Mapping:   2 H2|2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.660000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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