PrimePCR™ Probe Assay: Kcnma1, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $214.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0059551
Assay Design:   exonic
Chromosome Location:   14:23363870-23363988question
Amplicon Length:   89
Splice Variants Targeted:   ENSMUST00000179836 ENSMUST00000172099 ENSMUST00000065788 ENSMUST00000179097 ENSMUST00000177634 ENSMUST00000112423 ENSMUST00000163322 ENSMUST00000100831 ENSMUST00000074983

Gene Information

Description Not Available

Gene Symbol:   Kcnma1
Gene Name:   Potassium large conductance calcium-activated channel, subfamily M, alpha member 1
Aliases:   5730414M22Rik, BKCa, MaxiK, Slo, Slo1, mSlo, mSlo1
RefSeq:   NC_000080.6 NT_039606.8
Ensembl:   ENSMUSG00000063142
Entrez:   16531
UniGene:   Mm.343607
Chromosome Mapping:   14 A3|14

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.490000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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