PrimePCR™ Probe Assay: Fbxo18, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $214.00
Your Price:   Log In
 

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0058159
Assay Design:   exonic
Chromosome Location:   2:11748941-11749090question
Amplicon Length:   120
Splice Variants Targeted:   ENSMUST00000071564 ENSMUST00000114813 ENSMUST00000131893

Gene Information

Description Not Available

Gene Symbol:   Fbxo18
Gene Name:   F-box protein 18
Aliases:   AU015756, Fbh1, Fbx18
RefSeq:   NC_000068.7 NT_039202.8
Ensembl:   ENSMUSG00000058594
Entrez:   50755
UniGene:   Mm.24088
Chromosome Mapping:   2 A1|2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.510000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download