PrimePCR™ Probe Assay: Camk2b, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0058054
Assay Design:   exonic
Chromosome Location:   11:6015495-6028949question
Amplicon Length:   68
Splice Variants Targeted:   ENSMUST00000109813 ENSMUST00000109812 ENSMUST00000093355 ENSMUST00000066431 ENSMUST00000002817 ENSMUST00000019133 ENSMUST00000101585 ENSMUST00000101586 ENSMUST00000090443 ENSMUST00000109815

Gene Information

Description Not Available

Gene Symbol:   Camk2b
Gene Name:   Calcium/calmodulin-dependent protein kinase II, beta
Aliases:   Camk2d
RefSeq:   NC_000077.6 NT_039515.7
Ensembl:   ENSMUSG00000057897
Entrez:   12323
UniGene:   Mm.439733
Chromosome Mapping:   11 A1|11 0.5 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.510000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download