PrimePCR™ Probe Assay: Phc1, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $208.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0057643
Assay Design:   exonic
Chromosome Location:   6:122336928-122338493question
Amplicon Length:   80
Splice Variants Targeted:   ENSMUST00000161739 ENSMUST00000160696 ENSMUST00000159252 ENSMUST00000161054 ENSMUST00000160843 ENSMUST00000159384 ENSMUST00000161149 ENSMUST00000159657 ENSMUST00000161210 ENSMUST00000079560 ENSMUST00000081849 ENSMUST00000112600

Gene Information

Description Not Available

Gene Symbol:   Phc1
Gene Name:   Polyhomeotic-like 1 (Drosophila)
Aliases:   AW557034, Edr, Edr1, Mph1, Rae-28, rae28
RefSeq:   NC_000072.6 NT_039353.8
Ensembl:   ENSMUSG00000040669
Entrez:   13619
UniGene:   Mm.6822
Chromosome Mapping:   6 F3|6 57.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.640000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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