PrimePCR™ Probe Assay: Nkx1-1, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Nkx1-1, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Nkx1-1, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0056556
Assay Design:   exonic
Chromosome Location:   5:33431427-33431526question
Amplicon Length:   70
Splice Variants Targeted:   ENSMUST00000099552 ENSMUST00000101655 ENSMUST00000081318 ENSMUST00000066391 ENSMUST00000153425 ENSMUST00000140846 ENSMUST00000006397 ENSMUST00000039229 ENSMUST00000084806 ENSMUST00000113130 ENSMUST00000061013 ENSMUST00000044387 ENSMUST00000144079 ENSMUST00000074157 ENSMUST00000121875 ENSMUST00000128119 ENSMUST00000105286 ENSMUST00000001845 ENSMUST00000064454 ENSMUST00000111234 ENSMUST00000111236 ENSMUST00000118174 ENSMUST00000117422 ENSMUST00000119547 ENSMUST00000117488 ENSMUST00000105328 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Nkx1-1
Gene Name:   NK1 transcription factor related, locus 1 (Drosophila)
Aliases:   Nkx-1.1, Sax2
RefSeq:   NC_000071.6 NT_039305.8
Ensembl:   ENSMUSG00000029112
Entrez:   672284
Chromosome Mapping:   5 B1|5 18.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.480000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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