PrimePCR™ Probe Assay: Tacc2, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0055856
Assay Design:   exonic
Chromosome Location:   7:130729688-130729815question
Amplicon Length:   98
Splice Variants Targeted:   ENSMUST00000059145 ENSMUST00000084513 ENSMUST00000033141

Gene Information

This gene encodes a member of the transforming acidic coiled-coil (TACC) family of proteins. Members of this family are centrosomal proteins that interact with microtubules and tubulin. TACC proteins are thought to be involved in centrosome/mitotic spindle dynamics and gene regulation. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq Jul 2008]

Gene Symbol:   Tacc2
Gene Name:   Transforming, acidic coiled-coil containing protein 2
Aliases:   mKIAA4180
RefSeq:   NC_000073.6 NT_039433.8
Ensembl:   ENSMUSG00000030852
Entrez:   57752
UniGene:   Mm.86322
Chromosome Mapping:   7 F4|7

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.750000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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