PrimePCR™ Probe Assay: Crygf, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Crygf, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Crygf, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0055229
Assay Design:   exonic
Chromosome Location:   1:65926648-65926748question
Amplicon Length:   71
Splice Variants Targeted:   ENSMUST00000027089 ENSMUST00000114064 ENSMUST00000027090 ENSMUST00000061497 ENSMUST00000027082 ENSMUST00000114027 ENSMUST00000071880 ENSMUST00000081823 ENSMUST00000094135 ENSMUST00000126522 ENSMUST00000045068 ENSMUST00000107977 ENSMUST00000130235 ENSMUST00000021918 ENSMUST00000107865 ENSMUST00000167153 ENSMUST00000107864 ENSMUST00000107866 ENSMUST00000030306 ENSMUST00000026976 ENSMUST00000115513 ENSMUST00000115512 ENSMUST00000065143 ENSMUST00000124033 ENSMUST00000043441 ENSMUST00000087359 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Crygf
Gene Name:   Crystallin, gamma F
Aliases:   3110001K11Rik, Cryg-2, DGcry-2, Len-2
RefSeq:   NC_000067.6 NT_039170.8 NT_187020.1
Ensembl:   ENSMUSG00000025945
Entrez:   12969
Chromosome Mapping:   1 C3|1 32.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.680000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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