PrimePCR™ Probe Assay: Nudt3, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Nudt3, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Nudt3, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0055218
Assay Design:   exonic
Chromosome Location:   17:27583250-27623092question
Amplicon Length:   94
Splice Variants Targeted:   ENSMUST00000068726 ENSMUST00000111452 ENSMUST00000038648 ENSMUST00000034111 ENSMUST00000025050 ENSMUST00000114886 ENSMUST00000062397 ENSMUST00000176876 ENSMUST00000176458 ENSMUST00000080145 ENSMUST00000109694 ENSMUST00000032811 ENSMUST00000094617 ENSMUST00000110238 ENSMUST00000128646 ENSMUST00000148891 ENSMUST00000087445 ENSMUST00000032398 ENSMUST00000098457 ENSMUST00000150503 ENSMUST00000126532 ENSMUST00000179453 ENSMUST00000023220 ENSMUST00000029682 ENSMUST00000119084

Gene Information

Description Not Available

Gene Symbol:   Nudt3
Gene Name:   Nudix (nucleotide diphosphate linked moiety X)-type motif 3
Aliases:   1110011B09Rik, AA960325, Dipp
RefSeq:   NC_000083.6 NT_039649.8 NT_187005.1
Ensembl:   ENSMUSG00000024213
Entrez:   56409
Chromosome Mapping:   17 A3.3|17

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.820000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
10000088666 Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G Click to download
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3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
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