PrimePCR™ Probe Assay: Cplx2, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0054018
Assay Design:   exonic
Chromosome Location:   13:54381045-54381179question
Amplicon Length:   105
Splice Variants Targeted:   ENSMUST00000026985

Gene Information

Description Not Available

Gene Symbol:   Cplx2
Gene Name:   Complexin 2
Aliases:   921-L, AI413745, AW492120
RefSeq:   NC_000079.6 NT_039589.8
Ensembl:   ENSMUSG00000025867
Entrez:   12890
Chromosome Mapping:   13 B1|13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.630000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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