PrimePCR™ Probe Assay: Acvrl1, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Acvrl1, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Acvrl1, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0053847
Assay Design:   exonic
Chromosome Location:   15:101135305-101135394question
Amplicon Length:   60
Splice Variants Targeted:   ENSMUST00000153102 ENSMUST00000111215 ENSMUST00000075774 ENSMUST00000000542 ENSMUST00000120028 ENSMUST00000117984 ENSMUST00000119063 ENSMUST00000121718 ENSMUST00000124151 ENSMUST00000120754 ENSMUST00000066026 ENSMUST00000004208 ENSMUST00000109075 ENSMUST00000016397 ENSMUST00000033665 ENSMUST00000056355 ENSMUST00000039810 ENSMUST00000074834 ENSMUST00000023226 ENSMUST00000072692 ENSMUST00000169438 ENSMUST00000169714 ENSMUST00000169108 ENSMUST00000076442 ENSMUST00000171562 ENSMUST00000171634 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Acvrl1
Gene Name:   Activin A receptor, type II-like 1
Aliases:   AI115505, AI427544, Acvrlk1, Alk1
RefSeq:   NC_000081.6 NT_039621.8
Ensembl:   ENSMUSG00000000530
Entrez:   11482
Chromosome Mapping:   15|15 F3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.470000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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