This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: Mrvi1, Mouse
PrimePCR™ Template for Probe Assay: Mrvi1, Mouse
This gene is a putative tumor suppressor gene that is frequently disrupted by mouse AIDS-related virus (MRV). The encoded protein which is found in the membrane of the endoplasmic reticulum is similar to Jaw1 a lymphoid-restricted protein whose expression is downregulated during myeloid differentiation. Studies in mouse suggest that MRV integration at this locus induces myeloid leukemia by altering the expression of a gene important for myeloid cell growth and/or differentiation. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. Additional isoforms may arise through the use of alternative non-AUG (CUG) translation initiation site. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.