PrimePCR™ Probe Assay: Eya4, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0052727
Assay Design:   exonic
Chromosome Location:   10:23105902-23106039question
Amplicon Length:   108
Splice Variants Targeted:   ENSMUST00000092665 ENSMUST00000074366

Gene Information

Description Not Available

Gene Symbol:   Eya4
Gene Name:   Eyes absent 4 homolog (Drosophila)
Aliases:   B130023L16Rik
RefSeq:   NC_000076.6 NT_039492.8
Ensembl:   ENSMUSG00000010461
Entrez:   14051
Chromosome Mapping:   10 A3|10 18.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.780000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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