PrimePCR™ Probe Assay: Lass1, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0043689
Assay Design:   exonic
Chromosome Location:   8:70330956-70331165question
Amplicon Length:   180
Splice Variants Targeted:   ENSMUST00000025014 ENSMUST00000124516 ENSMUST00000018637 ENSMUST00000075734 ENSMUST00000108899 ENSMUST00000028341 ENSMUST00000129300 ENSMUST00000041362 ENSMUST00000110199 ENSMUST00000030372 ENSMUST00000046516 ENSMUST00000165819 ENSMUST00000140239

Gene Information

This gene encodes a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site that is cleaved to produce a mature protein containing seven conserved cysteine residues. The orthologous human protein has been shown to regulate C18-ceramide (N-stearoyl-sphinganine) synthesis. The protein is expressed from a bicistronic mRNA which also encodes growth differentiation factor 1. [provided by RefSeq Jul 2009]

Gene Symbol:   Lass1
Gene Name:   LAG1 homolog, ceramide synthase 1
Aliases:   CerS1, Uog-1, to
RefSeq:   NC_000074.6 NT_039462.8
Ensembl:   ENSMUSG00000087408
Entrez:   93898
Chromosome Mapping:   8 B3.3|8

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.780000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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