PrimePCR™ Probe Assay: Insl3, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Insl3, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Insl3, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0043652
Assay Design:   exonic
Chromosome Location:   8:71690439-71690561question
Amplicon Length:   93
Splice Variants Targeted:   ENSMUST00000005503 ENSMUST00000169684 ENSMUST00000020573 ENSMUST00000051995 ENSMUST00000034261 ENSMUST00000086101 ENSMUST00000144408 ENSMUST00000091851 ENSMUST00000159162 ENSMUST00000169266 ENSMUST00000163320 ENSMUST00000165239 ENSMUST00000005578 ENSMUST00000140164 ENSMUST00000149719 ENSMUST00000032815 ENSMUST00000085851 ENSMUST00000043552 ENSMUST00000017188 ENSMUST00000076532 ENSMUST00000167163 ENSMUST00000032719 ENSMUST00000161582 ENSMUST00000025639 ENSMUST00000037813 ENSMUST00000159991 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Insl3
Gene Name:   Insulin-like 3
Aliases:   Rlf, Rlnl
RefSeq:   NC_000074.6 NT_039462.8
Ensembl:   ENSMUSG00000079019
Entrez:   16336
Chromosome Mapping:   8 B3.3|8 33.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998000
y-intercept 39.850000
Efficiency 87

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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