PrimePCR™ Probe Assay: Hist1h4d, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0043334
Assay Design:   exonic
Chromosome Location:   13:23581618-23581767question
Amplicon Length:   120
Splice Variants Targeted:   ENSMUST00000102968 ENSMUST00000076617 ENSMUST00000179285 ENSMUST00000124516 ENSMUST00000018637 ENSMUST00000075734 ENSMUST00000171473 ENSMUST00000167824

Gene Information

Description Not Available

Gene Symbol:   Hist1h4d
Gene Name:   Histone cluster 1, H4d
Aliases:   Not Available
RefSeq:   NC_000079.6 NT_039578.8
Ensembl:   ENSMUSG00000061482
Entrez:   319156
Chromosome Mapping:   13 A2-A3|13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 37.540000
Efficiency 91

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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