PrimePCR™ Probe Assay: AK010878, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: AK010878, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: AK010878, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0043147
Assay Design:   exonic
Chromosome Location:   12:102757584-102757673question
Amplicon Length:   60
Splice Variants Targeted:   ENSMUST00000164267 ENSMUST00000006451 ENSMUST00000025521 ENSMUST00000087951 ENSMUST00000160973 ENSMUST00000162945 ENSMUST00000022566 ENSMUST00000048128 ENSMUST00000119606 ENSMUST00000121840 ENSMUST00000117956 ENSMUST00000169350 ENSMUST00000043676 ENSMUST00000114988 ENSMUST00000118487 ENSMUST00000161204 ENSMUST00000020655 ENSMUST00000109090 ENSMUST00000109091 ENSMUST00000148049 ENSMUST00000095725 ENSMUST00000035977 ENSMUST00000107635 ENSMUST00000033560 ENSMUST00000049614 ENSMUST00000092883 ENSMUS

Gene Information

This locus represents naturally occurring readthrough transcription between the neighboring AK010878 (cDNA sequence AK010878) and Moap1 (modulator of apoptosis 1) genes on chromosome 12. The readthrough transcript encodes a protein that shares sequence identity with the upstream gene product but its C-terminal region is distinct due to frameshifts relative to the downstream gene. [provided by RefSeq Dec 2011]

Gene Symbol:   AK010878
Gene Name:   CDNA sequence AK010878
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSMUSG00000041716
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.220000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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