PrimePCR™ Probe Assay: Eif4a2, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0041877
Assay Design:   exonic
Chromosome Location:   16:23113273-23113408question
Amplicon Length:   106
Splice Variants Targeted:   ENSMUST00000023599 ENSMUST00000077605 ENSMUST00000115341 ENSMUST00000168891

Gene Information

Description Not Available

Gene Symbol:   Eif4a2
Gene Name:   Eukaryotic translation initiation factor 4A2
Aliases:   4833432N07Rik, BM-010, Ddx2b, Eif4
RefSeq:   NC_000082.6 NT_039624.8
Ensembl:   ENSMUSG00000022884
Entrez:   13682
Chromosome Mapping:   16 B1|16 14.2 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.520000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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