PrimePCR™ SYBR® Green Assay: Vmn1r31, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency; Same primer pair as used in probe assay qMmuCEP0062929

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Vmn1r31, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Vmn1r31, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0061780
Assay Design:   exonic
Chromosome Location:   6:58472638-58472784question
Amplicon Length:   117
Splice Variants Targeted:   ENSMUST00000176838 ENSMUST00000096612 ENSMUST00000164307 ENSMUST00000079669 ENSMUST00000073384 ENSMUST00000078885 ENSMUST00000176376 ENSMUST00000177267 ENSMUST00000176395 ENSMUST00000164732 ENSMUST00000176085 ENSMUST00000175716 ENSMUST00000049694 ENSMUST00000095862 ENSMUST00000176874 ENSMUST00000168416 ENSMUST00000176177 ENSMUST00000176147 ENSMUST00000176023 ENSMUST00000177318 ENSMUST00000079584 ENSMUST00000175988 ENSMUST00000079678 ENSMUST00000074381 ENSMUST00000071414 ENSMUST00000176121 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Vmn1r31
Gene Name:   Vomeronasal 1 receptor 31
Aliases:   EG626828, Gm6709
RefSeq:   NC_000072.6 NT_039353.8
Ensembl:   ENSMUSG00000093379
Entrez:   626828
Chromosome Mapping:   6 B3|6

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.580000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download