PrimePCR™ SYBR® Green Assay: Mup14, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency; Same primer pair as used in probe assay qMmuCEP0062851

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Mup14, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Mup14, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0061702
Assay Design:   exonic
Chromosome Location:   4:61303817-61303946question
Amplicon Length:   100
Splice Variants Targeted:   ENSMUST00000075973 ENSMUST00000107517 ENSMUST00000079697 ENSMUST00000125282 ENSMUST00000166098 ENSMUST00000074700 ENSMUST00000107490 ENSMUST00000095058 ENSMUST00000163931 ENSMUST00000122381 ENSMUST00000118759 ENSMUST00000107506 ENSMUST00000122177 ENSMUST00000103012 ENSMUST00000084548 ENSMUST00000107499 ENSMUST00000098047 ENSMUST00000098046 ENSMUST00000084544 ENSMUST00000117932 ENSMUST00000072678 ENSMUST00000098042 ENSMUST00000075206 ENSMUST00000071005 ENSMUST00000095049 ENSMUST00000107483 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Mup14
Gene Name:   Major urinary protein 14
Aliases:   Gm13514, Mup12, OTTMUSG00000012493
RefSeq:   NC_000070.6 NT_187032.1
Ensembl:   ENSMUSG00000073830
Entrez:   100039116
Chromosome Mapping:   4 B3|4

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.100000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download