PrimePCR™ SYBR® Green Assay: Vmn1r59, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency; Same primer pair as used in probe assay qMmuCEP0062825

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Vmn1r59, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Vmn1r59, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0061676
Assay Design:   exonic
Chromosome Location:   7:5454261-5454403question
Amplicon Length:   113
Splice Variants Targeted:   ENSMUST00000098844 ENSMUST00000169556 ENSMUST00000170780 ENSMUST00000108569 ENSMUST00000074132 ENSMUST00000173782 ENSMUST00000172111 ENSMUST00000164880 ENSMUST00000173956 ENSMUST00000075085 ENSMUST00000078475 ENSMUST00000177783 ENSMUST00000177979

Gene Information

Description Not Available

Gene Symbol:   Vmn1r59
Gene Name:   Vomeronasal 1 receptor 59
Aliases:   V1rd10
RefSeq:   NC_000073.6 NT_187034.1
Ensembl:   ENSMUSG00000074401
Entrez:   404284
Chromosome Mapping:   7 A1|7

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 35.740000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download